Paraneoplastic Antigen MA2 (PNMA2) antibody

Antigen: Paraneoplastic Antigen MA2 (PNMA2)

Synonyms: MA2, MM2, RGAG2, KIAA0883, PNMA2, mKIAA0883, A830049P17Rik, MGC137318, DKFZp459C042

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN123377

Additional Information

Alternative name: PNMA2

UniProt: Q9UL42

Immunogen: Mixture of synthetic peptides containing amino acids 143-158 of human Ma2

Cross-Reactivity: Human

Description: In patients with cancer, symptoms of limbic and brain-stem dysfunction may result from a para- neoplastic disorder. Two proteins, Ma1 and Ma2, were discovered by presence of autoantibodies against them. Antibodies to Ma1 and Ma2 proteins identify a paraneoplastic disorder that affects the limbic system, brain stem, and cerebellum. These autoantibodies were used to clone Ma2, which codes for a protein (Ma2) that was recognized by serum from the 10 patients, but not by serum from 344 control subjects. Ma2 was selectively expressed by normal brain tissue and by the testicular tumors of the patients. Ma2 shares homology with Ma1, a brain-testis-cancer gene related to other para-neoplastic syndromes and tumors.

Application Details

Application Notes: Western blot: 1/500 - 1/1000. Recommended positive control: SK-N-SH. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Buffer: PBS containing 0.02% sodium azide and 0.1 mg/ml BSA

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of Ma2 in SK-N-SH cell lysate using ABIN123377 at 1:500.

Publications

Voltz, Gultekin, Rosenfeld et al.: "A serologic marker of paraneoplastic limbic and brain-stem encephalitis in patients with testicular cancer." in: The New England journal of medicine, Vol. 340, Issue 23, pp. 1788-95, 1999 (PubMed).

Debourdeau, Gligorov, Zammit: "A serologic marker of paraneoplastic limbic and brain-stem encephalitis in patients with testicular cancer." in: The New England journal of medicine, Vol. 341, Issue 19, pp. 1475-6, 1999 (PubMed).

Barnett, Prosser, Sutton et al.: "Paraneoplastic brain stem encephalitis in a woman with anti-Ma2 antibody." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 70, Issue 2, pp. 222-5, 2001 (PubMed).

Rosenfeld, Eichen, Wade et al.: "Molecular and clinical diversity in paraneoplastic immunity to Ma proteins." in: Annals of neurology, Vol. 50, Issue 3, pp. 339-48, 2001 (PubMed).