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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152,pSer156) antibody

Antigen

Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)
Synonyms MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530
Binding Site
Alternatives

pSer152,pSer156
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
2 references available
Catalog no. ABIN123379
Quantity 0.1 mL  (Variants)
Price 517.00 $   Plus shipping costs $45.00
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152,pSer156) antibody

Additional Information
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Alternative name MARCKS
UniProt P29966
Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho- Ser152 and phospho-Ser156 of rat MARCKS
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
Description Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Application Details
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Application Notes Western blot: 1:1000. Recommended Positive Control: rat brain (an ~87 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol
Storage For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Shelf Life: one year from despatch.
Research Area Cardiovascular
Restrictions For Research Use only

Publications
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Thelen, Rosen, Nairn et al.: "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane." in: Nature, Vol. 351, Issue 6324, pp. 320-2, 1991 (PubMed).

Spizz, Blackshear: "Protein kinase C-mediated phosphorylation of the myristoylated alanine-rich C-kinase substrate protects it from specific proteolytic cleavage." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 553-62, 1996 (PubMed).

Alternatives
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Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
Hosts Rabbit (174), Mouse (7), Goat (1), Rat (1)
Reactivities Human (166), Rat (Rattus) (131), Mouse (Murine) (126), Cow (Bovine) (77), Chicken (57), Dog (Canine) (55), Pig (Porcine) (38), Xenopus laevis (2), Zebrafish (Danio rerio) (2), Cat (Feline) (1), Rabbit (1)
Applications Western Blotting (WB) (104), Immunofluorescence (IF) (93), ELISA (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunoprecipitation (IP) (10), Immunoelectron Microscopy (IEM) (4), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes pSer162 (26), pSer170 (25), pSer152,pSer156 (16), pSer158 (10), C-Term (8), pSer152, pSer156 (5), pSer156,pSer152 (5), N-Term (3), Ser162 (3), pSer158,Internal Region (3), Internal Region (2), Ser158 (2), pSer163 (2), Phosphospecific (1), Ser152, Ser156 (1), pSer152 (1), pSer152, pSer153 (1), pSer159, pSer163 (1), phospho (1)