Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer156), (pSer152) antibody

Details for Product No. ABIN123379
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pSer156, pSer152
(26), (26), (25), (24), (23), (15), (9), (7), (6), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(195), (138), (128), (54), (40), (36), (25), (3), (3), (1), (1), (1)
(195), (17), (3)
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Western Blotting (WB)
(157), (59), (52), (40), (27), (26), (20), (4), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN123379
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Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho- Ser152 and phospho-Ser156 of rat MARCKS
Purification Sequential chromatography on phospho- and non-phosphopeptide affinity columns
Alternative Name MARCKS
Background Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
UniProt P29966
Research Area Cardiovascular
Application Notes Western blot: 1:1000. Recommended Positive Control: rat brain (an ~87 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol
Storage -20 °C
Storage Comment For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer156), (pSer152) antibody Western blot analysis using 10 ug of rat brain showing specific immunolabeling of MARCKS phosphorylated at Ser152/156 (Lane A). The labeling by the antibody to MARCKS phospho-Ser152/156 is specifically blocked by the Ser152/156 phosphopeptide used as antigen (Lane B). The corresponding non-phosphopeptide did not block the immunolabeling (not shown).
General Thelen, Rosen, Nairn et al.: "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane." in: Nature, Vol. 351, Issue 6324, pp. 320-2, 1991 (PubMed).

Spizz, Blackshear: "Protein kinase C-mediated phosphorylation of the myristoylated alanine-rich C-kinase substrate protects it from specific proteolytic cleavage." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 553-62, 1996 (PubMed).

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