Methyl-CpG Binding Domain Protein 1 (MBD1) antibody

Antigen: Methyl-CpG Binding Domain Protein 1 (MBD1)

Synonyms: RFT, PCM1, CXXC3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123380

Additional Information

Alternative name: MBD1

UniProt: Q9UIS9

Immunogen: Mixture of synthetic peptides corresponding to amino acids 98-113 and 391-405 of human MBD1 AA Sequence:RQVGPQSGEVRKEAPR / SESEDGAGSPPPYRR

Cross-Reactivity: Human

Description: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Specificity: This antibody is specific for MBD1. GenBank no ref|NP_056671.2|

Application Details

Application Notes: Western blot: 2.0 ug/ml. Recommended positive control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

Western blot analysis of MBD1 in HeLa cell lysate with anti-MBD1 pcAb. A protein band with an approximate molecular weight of 55 kDa was detected.

Publications

Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).

Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Petronzelli, Riccio, Markham et al.: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

Bader, Walker, Harrison: "Most microsatellite unstable sporadic colorectal carcinomas carry MBD4 mutations." in: British journal of cancer, Vol. 83, Issue 12, pp. 1646-9, 2001 (PubMed).