Methyl CpG Binding Protein 2 (Rett Syndrome) (MECP2) (AA 11-25), (AA 181-195) antibody

Details for Product No. ABIN123385
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Antigen
Epitope
AA 11-25, AA 181-195
(18), (15), (13), (9), (7), (7), (6), (6), (6), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(115), (37), (31), (15), (12), (12), (12), (2), (2), (1)
Host
Rabbit
(108), (11), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(85), (60), (25), (21), (17), (13), (10), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN123385
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Immunogen Mixture of synthetic peptides corresponding to amino acids 11-25 and 181-195 of human MeCP2
Purification Protein G Chromatography
Alternative Name MeCP2
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).
UniProt P51608
Research Area Neurology
Application Notes Western blot: 2.0 ug/ml. Recommended Positive Control: HeLa. In HeLa, a 53 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Methyl CpG Binding Protein 2 (Rett Syndrome) (MECP2) (AA 11-25), (AA 181-195) antibody Western blot analysis of MeCP2 in HeLa cell lysate with anti- MeCP2 pcAb ABIN123385. A protein band of approximate molecular weight of 53 kDa was detected.
General Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).

Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Petronzelli, Riccio, Markham et al.: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

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