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Histone H4 Transcription Factor (HINFP) (AA 180-194), (AA 331-346), (AA 371-388) antibody

Details for Product No. ABIN123388
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Antigen
Epitope
AA 180-194, AA 331-346, AA 371-388
(8), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(22), (5), (3), (2), (1), (1), (1)
Host
Rabbit
(19), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (11), (2), (2)
Pubmed 2 references available
Quantity 0.2 mL
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN123388
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Immunogen Mixture of synthetic peptides corresponding to amino acid residues, 180-194, 331-346, and 371- 388 of human MIZF
Purification Purified
Alternative Name MIZF
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. Recently, using MBD2 as bait in two- hybrid screening, a novel zing finger protein, called MIZF has been identified (3). This MBD2- binding zinc finger (MIZF) protein represses transcription by associating with MBD2 and a histone deacetylase complex. It is ubiquitously expressed in all human tissues.
UniProt Q9BQA5
Application Notes Western blot: 1/500. Recommended positive control: MOLT-4. In MOLT-4, a 64 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Histone H4 Transcription Factor (HINFP) (AA 180-194), (AA 331-346), (AA 371-388) antibody Western blot analysis of MIZF on MOLT-4 cell lysate. Ten microgram of protein was loaded per lane of a mini gel. The antibody was used at 1:500 dilution.
General Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Sekimata, Takahashi, Murakami-Sekimata et al.: "Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2." in: The Journal of biological chemistry, Vol. 276, Issue 46, pp. 42632-8, 2001 (PubMed).

Validation Images
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