MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody

Antigen: MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A)

Synonyms: ATLD, HNGS1, MRE11, MRE11B, Mre11, zgc:64018, MRE11A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123393

Additional Information

Alternative name: MRE11

UniProt: P49959

Immunogen: Synthetic peptide of human MRE11

Cross-Reactivity: Human

Description: MRE11 in yeast belongs to RAD50 epistasis group gene, MRE11, blocks meiotic recombination and play roles in non-homologous end-joining (NHEJ) DNA double-strand break (DSB) repair pathways. Human MRE11 shares approximately 50% identity at the amino acid level with its yeast counterpart in the N-terminal half of the protein (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, MRE11, and the NBS1 gene product, p95 (NBS protein, nibrin) (2). In addition function of MRE11 is also linked to ataxia telangiectasia mutated (ATM) protein kinase (3), TTAGGG repeat binding factor2 (TRF2) (4), and Ku70 (5). A mutation of the hMRE11 gene may be important in a new ataxia telangiectasia-like disorder (ATLD) (6). MRE11 is expressed at the highest levels in proliferating tissues.

Specificity: This antibody is specific for MRE11.

Application Details

Application Notes: Western blot (1-2 ug/ml). Recommended Positive Control: 293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS and 0.02 % (w/v) sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis for Mre11 in 293 cells: 10 microgram per lane 293 cell lysate was resolved by SDS-PAGE and the blot was probed with 2 ug/ml anti-Mre11 antibody (Cat. # ABIN123393).

Publications

Petrini, Walsh, DiMare et al.: "Isolation and characterization of the human MRE11 homologue." in: Genomics, Vol. 29, Issue 1, pp. 80-6, 1996 (PubMed).

Dong, Zhong, Chen: "The Nijmegen breakage syndrome protein is essential for Mre11 phosphorylation upon DNA damage." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19513-6, 1999 (PubMed).

Petrini: "The Mre11 complex and ATM: collaborating to navigate S phase." in: Current opinion in cell biology, Vol. 12, Issue 3, pp. 293-6, 2000 (PubMed).

Li, Zhu, Waterton et al.: "Improved 3D quantitative mapping of blood volume and endothelial permeability in brain tumors." in: Journal of magnetic resonance imaging : JMRI, Vol. 12, Issue 2, pp. 347-57, 2000 (PubMed).