Toll-Like Receptor 2 (TLR2) antibody

Antigen: Toll-Like Receptor 2 (TLR2)

Synonyms: TIL4, CD282, Ly105, TLR2, TLR2A, chTLR2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chimpanzee, Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123396

Additional Information

Alternative name: CD282 / TLR2

UniProt: O60603

Immunogen: Amino acids 375-390 of mouse TLR2

Cross-Reactivity: Chimpanzee, Human, Mouse (Murine), Rat (Rattus)

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM- CSF, IL-1, and IL-10 up-regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Application Details

Application Notes: Western blot: 1/500 - 1/2000. Recommended positive control: RAW. In RAW, a 86 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of mTLR2 in 15 ugs of Raw cell lysate using ABIN123396 at 1:2000 dilution.

Publications

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Chuang, Ulevitch: "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9." in: European cytokine network, Vol. 11, Issue 3, pp. 372-8, 2000 (PubMed).

Flo, Halaas, Torp et al.: "Differential expression of Toll-like receptor 2 in human cells." in: Journal of leukocyte biology, Vol. 69, Issue 3, pp. 474-81, 2001 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).