Nuclear Factor-KB P65 (NFkBP65) (AA 538-546) antibody

Details for Product No. ABIN123408
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Antigen
Epitope
AA 538-546
(69), (59), (50), (46), (39), (34), (34), (33), (32), (32), (29), (22), (21), (12), (12), (8), (7), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(900), (446), (356), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Rabbit
(842), (69), (6), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(24), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
Western Blotting (WB)
(690), (365), (300), (186), (151), (126), (100), (54), (39), (21), (19), (19), (18), (12), (7), (5), (2), (1)
Pubmed 1 reference available
Quantity 0.2 mL
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Catalog No. ABIN123408
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Immunogen Synthetic peptide corresponding to aa 538-546 of human p65
Purification Protein G affinity Chromatography
Alternative Name RELA / NF-kB p65
Background NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
UniProt Q04206
Application Notes Western blot analysis: 1:500-1:1000. Recommended positive control: HeLa or RAW cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide and 0.1 mg/ml BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Nuclear Factor-KB P65 (NFkBP65) (AA 538-546) antibody Western blot analysis of p65 in 15 ugs of HeLa cell lysate using ABIN123408 at 1:1000 dilution.
General Ganchi, Sun, Greene et al.: "I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding." in: Molecular biology of the cell, Vol. 3, Issue 12, pp. 1339-52, 1993 (PubMed).

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