RELA / NF-kB p65 antibody

Antigen: RELA / NF-kB p65

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN123408

Additional Information

Alternative name:

UniProt: Q04206

Immunogen: Synthetic peptide corresponding to aa 538-546 of human p65

Cross-Reactivity: Human, Mouse (Murine)

Description: NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Application Details

Application Notes: Western blot analysis: 1:500-1:1000. Recommended positive control: HeLa or RAW cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS containing 0.02% sodium azide and 0.1 mg/ml BSA

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of p65 in 15 ugs of HeLa cell lysate using ABIN123408 at 1:1000 dilution.

Publications

Ganchi, Sun, Greene et al.: "I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding." in: Molecular biology of the cell, Vol. 3, Issue 12, pp. 1339-52, 1993 (PubMed).