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GATAD2A / Hp66 alpha antibody
Human, Mouse (Murine), Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|4 references available|
|Quantity||0.2 mL (Variants)|
|Price||319.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Immunogen||Synthetic peptide corresponding to aa 137-150 of mouse p66a|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||Methylation of cytosine at CpG dinucleotides is a common feature of many highereukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histonedeacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism ofmethylated DNA silencing. P66, a zinc finger-containing protein is one of the componentsof the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different formsof the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 andCR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2plays an important role in targeting p66 and MBD3 to specific loci (4).|
|Characteristics||Synonyms: GATA zinc finger domain-containing protein 2A, Transcriptional repressor p66-alpha|
|Application Notes||Western blot: 1: 500-1: 1000. Recommended Positive Control: HeLa or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of p66a in 15 ugs of HeLa cell lysate using IMG-509 at 1:500 dilution.|
Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).
Xue, Wong, Moreno et al.: "NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities." in: Molecular cell, Vol. 2, Issue 6, pp. 851-61, 1999 (PubMed).
Zhang, Ng, Erdjument-Bromage et al.: "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation." in: Genes & development, Vol. 13, Issue 15, pp. 1924-35, 1999 (PubMed).
Feng, Cao, Xia et al.: "Identification and functional characterization of the p66/p68 components of the MeCP1 complex." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 536-46, 2001 (PubMed).
|Reactivities||Mouse (Murine) (3), Human (2), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Rat (Rattus) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (3), ELISA (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|