GATA Zinc Finger Domain Containing 2B (GATAD2B) antibody

Antigen: GATA Zinc Finger Domain Containing 2B (GATAD2B)

Synonyms: P66beta, FLJ37346, KIAA1150, MGC138257, MGC138285, RP11-216N14.6, RGD1308533, GATAD2B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN123410

Additional Information

Alternative name: GATAD2B / Hp66 beta

Gene ID: 229542

UniProt: Q8VHR5

Immunogen: Synthetic peptide corresponding to aa 445-460 of mouse p66b

Cross-Reactivity: Human, Mouse (Murine)

Description: Methylation of cytosine at CpG dinucleotides is a common feature of many highereukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histonedeacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism ofmethylated DNA silencing. P66, a zinc finger-containing protein is one of the componentsof the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different formsof the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 andCR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2plays an important role in targeting p66 and MBD3 to specific loci (4).

Characteristics: Synonyms: p66/p68, KIAA1150, Transcriptional repressor p66-beta, GATA zinc fingerdomain-containing protein 2B

Specificity: This antibody is specific for p66b. Species: Human, Mouse. Others not tested.

Application Details

Application Notes: Western blot: 2-4 ug/ml. Recommended positive control: HeLa or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Serum

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of p66b in 15 ugs of Raw cell lysate using ABIN123410 at 1:500 dilution.

Publications

Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).

Xue, Wong, Moreno et al.: "NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities." in: Molecular cell, Vol. 2, Issue 6, pp. 851-61, 1999 (PubMed).

Zhang, Ng, Erdjument-Bromage et al.: "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation." in: Genes & development, Vol. 13, Issue 15, pp. 1924-35, 1999 (PubMed).

Feng, Cao, Xia et al.: "Identification and functional characterization of the p66/p68 components of the MeCP1 complex." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 536-46, 2001 (PubMed).