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|+1 888 205 9894 (TF)|
GATA Zinc Finger Domain Containing 2B (GATAD2B) antibody
|Synonyms||P66beta, FLJ37346, KIAA1150, MGC138257, MGC138285, RP11-216N14.6, RGD1308533, GATAD2B|
Alternatives Western Blotting (WB)
|4 references available|
|Quantity||0.2 ml (0.5 mg/ml)|
|Price||269.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||GATAD2B / Hp66 beta|
|Immunogen||Synthetic peptide corresponding to aa 445-460 of mouse p66b|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Methylation of cytosine at CpG dinucleotides is a common feature of many highereukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histonedeacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism ofmethylated DNA silencing. P66, a zinc finger-containing protein is one of the componentsof the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different formsof the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 andCR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2plays an important role in targeting p66 and MBD3 to specific loci (4).|
|Characteristics||Synonyms: p66/p68, KIAA1150, Transcriptional repressor p66-beta, GATA zinc fingerdomain-containing protein 2B|
|Specificity||This antibody is specific for p66b. Species: Human, Mouse. Others not tested.|
|Application Notes||Western blot: 2-4 ug/ml. Recommended positive control: HeLa or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2% gelatin and 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of p66b in 15 ugs of Raw cell lysate using ABIN123410 at 1:500 dilution.|
Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).
Xue, Wong, Moreno et al.: "NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities." in: Molecular cell, Vol. 2, Issue 6, pp. 851-61, 1999 (PubMed).
Zhang, Ng, Erdjument-Bromage et al.: "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation." in: Genes & development, Vol. 13, Issue 15, pp. 1924-35, 1999 (PubMed).
Feng, Cao, Xia et al.: "Identification and functional characterization of the p66/p68 components of the MeCP1 complex." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 536-46, 2001 (PubMed).
|Hosts||Rabbit (15), Goat (2), Mouse (2)|
|Reactivities||Human (16), Mouse (Murine) (6), Cow (Bovine) (3), Dog (Canine) (3), Rat (Rattus) (3), Zebrafish (1)|
|Applications||Western Blotting (WB) (16), ELISA (6), Immunoprecipitation (IP) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2)|
|Epitopes||N-Term (4), AA 445-460 (2), C-Term (2), AA 1-50 (1), AA 176-191 (1), AA 50-100 (1), AA 543-593 (1)|