GATAD2B / Hp66 beta (AA 445-460) antibody

Details for Product No. ABIN123410
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Antigen
Epitope
AA 445-460
Reactivity
Human, Mouse (Murine)
(1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
Western Blotting (WB)
(1)
Pubmed 4 references available
Catalog no. ABIN123410
Quantity 0.2 mL
Price
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Immunogen Synthetic peptide corresponding to aa 445-460 of mouse p66b
Characteristics Synonyms: p66/p68, KIAA1150, Transcriptional repressor p66-beta, GATA zinc fingerdomain-containing protein 2B
Purification Protein G Chromatography
Background Methylation of cytosine at CpG dinucleotides is a common feature of many highereukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histonedeacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism ofmethylated DNA silencing. P66, a zinc finger-containing protein is one of the componentsof the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different formsof the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 andCR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2plays an important role in targeting p66 and MBD3 to specific loci (4).
Gene ID 229542
UniProt Q8VHR5
Application Notes Western blot: 2-4 ug/ml. Recommended positive control: HeLa or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-GATAD2B / Hp66 beta (AA 445-460) antibody Western blot analysis of p66b in 15 ugs of Raw cell lysate using ABIN123410 at 1:500 dilution.
General Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).

Xue, Wong, Moreno et al.: "NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities." in: Molecular cell, Vol. 2, Issue 6, pp. 851-61, 1999 (PubMed).

Zhang, Ng, Erdjument-Bromage et al.: "Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation." in: Genes & development, Vol. 13, Issue 15, pp. 1924-35, 1999 (PubMed).

Feng, Cao, Xia et al.: "Identification and functional characterization of the p66/p68 components of the MeCP1 complex." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 536-46, 2001 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1)
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