Protein Inhibitor of Activated STAT, 3 (PIAS3) antibody

Antigen: Protein Inhibitor of Activated STAT, 3 (PIAS3)

Synonyms: ZMIZ5, FLJ14651, Pias3l, MGC27936, MGC81944, PIAS3, MGC165870

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN123411

Additional Information

Alternative name: PIAS3

UniProt: Q9Y6X2

Immunogen: Mixture of synthetic peptides corresponding to amino acids 44-60 and 187-198 of human PIASg

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIASgamma gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.

Application Details

Application Notes: Immunoflourescence (see Ihara et al, 2005 for details). Western blot: 1- 2 ug/ml. Recommended Positive Control: Jurkat. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin, 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of PIASgamma in Jurkat cell lysate using ABIN123411 at 2 ug/ml.

Publications

Uddin, Chamdin, Platanias: "Interaction of the transcriptional activator Stat-2 with the type I interferon receptor." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 24627-30, 1995 (PubMed).

Schindler, Darnell: "Transcriptional responses to polypeptide ligands: the JAK-STAT pathway." in: Annual review of biochemistry, Vol. 64, pp. 621-51, 1995 (PubMed).

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997 (PubMed).

Sturm, Kollerits, Neyer et al.: "Uric acid as a risk factor for progression of non-diabetic chronic kidney disease? The Mild to Moderate Kidney Disease (MMKD) Study." in: Experimental gerontology, 2008 (PubMed).