Protein Arginine Methyltransferase 6 (PRMT6) antibody

Antigen: Protein Arginine Methyltransferase 6 (PRMT6)

Synonyms: CG9927, DART6, DmelCG9927, HRMT1L6, FLJ10559, FLJ51477, Hrmt1l6, AW124876, BB233495, hrmt6, hrmt1l6, im:6908706, PRMT6, MGC83989, prmt6, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6, ATPRMT6, PROTEIN ARGININE METHYLTRANSFERASE 6, DKFZp459B1431

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123417

Additional Information

Alternative name: PRMT6 / HRMT1L6

UniProt: Q96LA8

Immunogen: Synthetic peptides corresponding to amino acids 23-43 of human PRMT6. AA Sequence: CEEDGAEREAALERPRRTKRER

Cross-Reactivity: Human, Mouse (Murine)

Description: Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N- methyltransferase (PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42 kDa consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG- monomethylarginine and asymmetric >NG,NG-dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activity. GFP- fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus. Alternate Names: PRMT6, HRMT1L6, Protein arginine N-methyltransferase 6, , Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6.

Synonyms: CG9927, DART6, DmelCG9927, HRMT1L6, FLJ10559, FLJ51477, Hrmt1l6, AW124876, BB233495, hrmt6, hrmt1l6, im:6908706, PRMT6, MGC83989, prmt6, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6, ATPRMT6, PROTEIN ARGININE METHYLTRANSFERASE 6, DKFZp459B1431

Application Details

Application Notes: Western blot: 1/500 - 1/1000. HeLa cell lysate or RAW can be used as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS, containing 0.05% BSA and 0.05% sodium azide.

Storage: Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of PRMT6 in cell lysates from HeLa cells using ABIN123417 at 1:500 dilution. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.

Publications

Chen, Ma, Hong et al.: "Regulation of transcription by a protein methyltransferase." in: Science (New York, N.Y.), Vol. 284, Issue 5423, pp. 2174-7, 1999 (PubMed).

Frankel, Yadav, Lee et al.: "The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3537-43, 2002 (PubMed).