PUMA antibody

Antigen: PUMA

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123420

Additional Information

Alternative name:

UniProt: Q9BXH1

Immunogen: A portion of amino acids 150-193 of human PUMA a

Cross-Reactivity: Human, Mouse (Murine)

Description: A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Application Details

Application Notes: IHC (paraffin): see Karst et al, 2005. Western blots (1:500 - 1:1,000). Recommended Positive Control: Jurkat or NIH 3T3 cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin, 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, Apoptosis/Necrosis, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis for Puma (CT) using ABIN123420 at 1:500 against 30 ug/lane of K562 whole cell lysate.

Publications

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).