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Details for Product No. ABIN123421

PUMA (N-Term) antibody

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Antigen
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN123421
Quantity 0.1 mg
Price
654.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptides of human PUMAa (NT)
Purification Protein G Chromatography
Background A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Application Notes IHC: 10 ug/ml. Western blots (0.5 - 1.0 ug/ml). Recommended Positive Control: Western blot: 3T3 cells, K562 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.02% Sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Alternatives for antigen "PUMA", type "Antibodies"
Hosts (52), (32), (1)
Reactivities (84), (37), (37), (1), (1)
Applications (81), (44), (13), (13), (12), (10), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1)
Epitopes (28), (12), (10), (7), (2), (1), (1), (1)
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