PUMA antibody

Antigen: PUMA

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN123421

Additional Information

Alternative name:

UniProt: Q9BXH1

Immunogen: Synthetic peptides of human PUMAa (NT)

Cross-Reactivity: Human, Mouse (Murine)

Description: A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Application Details

Application Notes: IHC: 10 ug/ml. Western blots (0.5 - 1.0 ug/ml). Recommended Positive Control: Western blot: 3T3 cells, K562 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, Apoptosis/Necrosis, Metabolism

Restrictions: For Research Use only

Images

IHC of PUMA in human breast carcinoma with anti-PUMA (NT) at 10 µg/ml.

Publications

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).