Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP) (AA 212-235) antibody

Details for Product No. ABIN123425
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Antigen
Epitope
AA 212-235
(10), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(33), (6), (4), (2), (1), (1)
Host
Rabbit
(25), (7), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(33), (15), (9), (6), (5), (4), (4), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN123425
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Immunogen Synthetic peptides corresponding amino acids 212-235 of human Rap1
Purification Protein G Chromatography
Alternative Name TERF2IP / RAP1
Background Human Rap1 was the first identified ortholog of the yeast telomeric protein scRap1, which shares 3 conserved sequence motifs in common. HRap1 is located at telomeres and affects telomere length by being recruited to telomeres by TRF2 instead of directly binding to the telomere like scRap1. The hRap1 cDNA codes for a protein of 399 amino acids with approximate molecular weight of 47 kDa. The hRap1 protein does not interact with TRF1. TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length(1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (5). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (4). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (4). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3).
UniProt Q9NYB0
Research Area Cell Cycle, Signaling
Application Notes Western blot: 2.0 ug/ml. Recommended positive control: 293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide and 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP) (AA 212-235) antibody Detection of hRap1 in 293 cells: Two microgram of anti-Rap1 (ABIN123425) was used to detect hRap1 in 293 cells. A protein band with an approximate molecular weight of 50 kDa was detected.
General Broccoli, Smogorzewska, Chong et al.: "Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2." in: Nature genetics, Vol. 17, Issue 2, pp. 231-5, 1997 (PubMed).

van Steensel, Smogorzewska, de Lange: "TRF2 protects human telomeres from end-to-end fusions." in: Cell, Vol. 92, Issue 3, pp. 401-13, 1998 (PubMed).

Karlseder, Broccoli, Dai et al.: "p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2." in: Science (New York, N.Y.), Vol. 283, Issue 5406, pp. 1321-5, 1999 (PubMed).

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