Sirtuin 5 (SIRT5) (AA 30-46) antibody

Details for Product No. ABIN123439
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Antigen
Epitope
AA 30-46
(22), (7), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(88), (35), (26), (4), (3), (2), (2), (1), (1)
Host
Rabbit
(68), (23), (8), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(86), (38), (21), (17), (16), (12), (10), (4), (4), (3)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN123439
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Immunogen Synthetic peptide corresponding to amino acids 30-46 of human SIRT5
Characteristics Synonyms: SIRT-5, SIR2-like protein 5, SIR2L5, sirtuin type 5, sirtuin 5, sirtuin-5, sirtuin (silent matingtype information regulation 2 homolog) 5 (S. cerevisiae), NAD-dependent deacetylasesirtuin-5
Purification Protein G Chromatography
Alternative Name SIRT5
Background SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and arepresent in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain,which contains a series of sequence motifs conserved in organisms ranging from bacteriato humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD andpossibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yetcompletely understood but recent reports of histone-activated Sir2-mediated NADmetabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possiblecoupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the Nepsilon-acetyl-lysine groups of acetylated histones.
Gene ID 23408
UniProt Q9NXA8
Research Area Chromatin and Nuclear Signaling, Proteases
Application Notes Western blot (1 - 3 ug/ml, rec. positive control: human, mouse or rat intestine). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Sirtuin 5 (SIRT5) (AA 30-46) antibody Western blot analysis of Sirt5 in A) human, B) mouse and C) rat intestine cell lysate using ABIN123439 at 2 ug/ml.
General Frye: "Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity." in: Biochemical and biophysical research communications, Vol. 260, Issue 1, pp. 273-9, 1999 (PubMed).

Landry, Sutton, Tafrov et al.: "The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 5807-11, 2000 (PubMed).

Frye: "Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins." in: Biochemical and biophysical research communications, Vol. 273, Issue 2, pp. 793-8, 2000 (PubMed).

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