Sirtuin 5 (SIRT5) antibody

Antigen: Sirtuin 5 (SIRT5)

Synonyms: SIR2L5, FLJ36950, AV001953, 0610012J09Rik, 1500032M05Rik, MGC93823, zgc:92288, SIRT5, MGC128489, sir2l5, DKFZp468D0719

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123439

Additional Information

Alternative name: SIRT5

Gene ID: 23408

UniProt: Q9NXA8

Immunogen: Synthetic peptide corresponding to amino acids 30-46 of human SIRT5

Cross-Reactivity: Human

Description: SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and arepresent in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain,which contains a series of sequence motifs conserved in organisms ranging from bacteriato humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD andpossibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yetcompletely understood but recent reports of histone-activated Sir2-mediated NADmetabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possiblecoupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the Nepsilon-acetyl-lysine groups of acetylated histones.

Characteristics: Synonyms: SIRT-5, SIR2-like protein 5, SIR2L5, sirtuin type 5, sirtuin 5, sirtuin-5, sirtuin (silent matingtype information regulation 2 homolog) 5 (S. cerevisiae), NAD-dependent deacetylasesirtuin-5

Specificity: This antibody is specific for SIRT5. Species: Human, Mouse, Rat. Others not tested.

Application Details

Application Notes: Western blot (1 - 3 ug/ml, rec. positive control: human, mouse or rat intestine). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Proteases

Restrictions: For Research Use only

Images

Western blot analysis of Sirt5 in A) human, B) mouse and C) rat intestine cell lysate using ABIN123439 at 2 ug/ml.

Publications

Frye: "Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity." in: Biochemical and biophysical research communications, Vol. 260, Issue 1, pp. 273-9, 1999 (PubMed).

Landry, Sutton, Tafrov et al.: "The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 5807-11, 2000 (PubMed).

Frye: "Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins." in: Biochemical and biophysical research communications, Vol. 273, Issue 2, pp. 793-8, 2000 (PubMed).