Sirtuin 7 (SIRT7) antibody

Antigen: Sirtuin 7 (SIRT7)

Synonyms: SIR2L7, MGC126840, MGC126842, SIRT7, MGC142712, LOC796135

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123440

Additional Information

Alternative name: SIRT7

Gene ID: 51547

UniProt: Q9NRC8

Immunogen: Mixture of synthetic peptides corresponding to amino acids 35-51 and 361-377 of humanSIRT7

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: SIRT7 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and arepresent in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain,which contains a series of sequence motifs conserved in organisms ranging from bacteriato humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD andpossibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yetcompletely understood but recent reports of histone-activated Sir2-mediated NADmetabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possiblecoupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the Nepsilon-acetyl-lysine groups of acetylated histones

Characteristics: Synonyms: SIRT-7, SIR2-like protein 7, SIR2L7, sirtuin type 7, sirtuin 7, sirtuin-7, sirtuin (silent matingtype information regulation 2, S.cerevisiae, homolog) 7, NAD-dependent deacetylasesirtuin-7

Specificity: This antibody is specific for SIRT7. Expected to cross-react with Mouse (100% identity with immunogen), Human (100%identity with immunogen) and Rat (88% identity with immunogen) due to sequencehomology. Species: Human,mouse, rat. Others not tested.

Application Details

Application Notes: Western blot (1 - 3 ug/ml). Recommended Positive Control: human liver or PBMC. There is no corresponding 40 kD band seen in mouse or rat liver cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2 % gelatin, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, Chromatin Binding Proteins, Transcription Factors, Proteases

Restrictions: For Research Use only

Images

Western blot analysis for SIRT7 using ABIN123440 at 1 ug/ml against 15 ug of human liver lysate.

Publications

Frye: "Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity." in: Biochemical and biophysical research communications, Vol. 260, Issue 1, pp. 273-9, 1999 (PubMed).

Landry, Sutton, Tafrov et al.: "The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 5807-11, 2000 (PubMed).

Frye: "Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins." in: Biochemical and biophysical research communications, Vol. 273, Issue 2, pp. 793-8, 2000 (PubMed).