Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 199-212), (AA 226-239) antibody

Details for Product No. ABIN123441
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Antigen
Synonyms Smac, DFNA64, SMAC, DBO, XSmac, smac, smac3, diablo-s
Epitope
AA 199-212, AA 226-239
(27), (8), (7), (4), (3), (3), (3), (2), (2), (2), (1)
Reactivity
Human
(122), (54), (47), (14), (14), (13), (13), (2), (1)
Host
Rabbit
(111), (20), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(122), (42), (39), (35), (15), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN123441
Quantity 0.1 mg
Price
456.50 $   Plus shipping costs $45.00
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Immunogen Two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac
Purification Protein G Chromatography
Alternative Name DIABLO / SMAC
Background Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa (3).
UniProt Q9NR28
Application Notes IHC (paraffin): please Ren et al., 2006 for details. Western blot: 0.5 - 2.0 ug/ml. Recommended Positive Control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. .
Expiry Date 12 months
Supplier Images
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 199-212), (AA 226-239) antibody Western blot analysis of Smac/DIABLO in HeLa cell
General Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

Mizutani, Nakanishi, Yamamoto et al.: "Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 23, Issue 3, pp. 448-54, 2005 (PubMed).

Berthier, Lemaire-Ewing, Prunet et al.: "7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways." in: The FEBS journal, Vol. 272, Issue 12, pp. 3093-104, 2005 (PubMed).

Qin, Ziffra, Stennett et al.: "Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells." in: Cancer research, Vol. 65, Issue 14, pp. 6282-93, 2005 (PubMed).

Hosts (111), (20), (5)
Reactivities (122), (54), (47), (14), (14), (13), (13), (2), (1)
Applications (122), (42), (39), (35), (15), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (27), (8), (7), (4), (3), (3), (3), (2), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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