Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody

Antigen: Second Mitochondria-Derived Activator of Caspase (DIABLO)

Synonyms: SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049, smac, smac3, diablo-s, XSmac, diablo

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123441

Additional Information

Alternative name: DIABLO / SMAC

UniProt: Q9NR28

Immunogen: Two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac

Cross-Reactivity: Human

Description: Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa (3).

Specificity: This antibody is specific for Smac/DIABLO. Genbank accession no. AAF87716

Application Details

Application Notes: IHC (paraffin): please Ren et al., 2006 for details. Western blot: 0.5 - 2.0 ug/ml. Recommended Positive Control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. .

Restrictions: For Research Use only

Images

Western blot analysis of Smac/DIABLO in HeLa cell

Publications

Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

Mizutani, Nakanishi, Yamamoto et al.: "Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 23, Issue 3, pp. 448-54, 2005 (PubMed).

Berthier, Lemaire-Ewing, Prunet et al.: "7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways." in: The FEBS journal, Vol. 272, Issue 12, pp. 3093-104, 2005 (PubMed).

Qin, Ziffra, Stennett et al.: "Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells." in: Cancer research, Vol. 65, Issue 14, pp. 6282-93, 2005 (PubMed).