SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) antibody

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1)

Synonyms: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123454

Additional Information

Alternative name: SUMO1

UniProt: P63165

Immunogen: Synthetic peptide of human SUMO1

Cross-Reactivity: Human, Mouse (Murine)

Description: Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Synonyms: MGC109561, UBL1, zgc:65934, zgc:85634, hm:zeh0670, wu:fb74c02, wu:fd12c02, SUMO1, ubl1, sumo-1, xsumo1, sumo1-b, xsumo-1, MGC85025, ATSUMO1, F10M23.180, F10M23_180, SMALL UBIQUITIN-LIKE MODIFIER 1, SUM1, SUMO 1, pigSUMO1, sumo1, DKFZp469M0816, GMP1, PIC1, SMT3, DAP-1, OFC10, SENP2, SMT3C, SMT3H3, SUMO-1, Ubl1, SMTP3, Smt3C, SENTRIN, MGC103203

Application Details

Application Notes: Western blotting (2-3 ug/ml, Recommended positive control: HeLa or NIH 3T3, A 15 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS containing 0.09% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Proteases, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of SUMO1 in 15 ugs of HeLa cell lysate using ABIN123454 at 2 ug/ml.

Publications

Müller, Hoege, Pyrowolakis et al.: "SUMO, ubiquitin's mysterious cousin." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 3, pp. 202-10, 2001 (PubMed).

Kahyo, Nishida, Yasuda: "Involvement of PIAS1 in the sumoylation of tumor suppressor p53." in: Molecular cell, Vol. 8, Issue 3, pp. 713-8, 2001 (PubMed).