SUMO2/3 antibody

Antigen: SUMO2/3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123455

Additional Information

Alternative name:

Gene ID: 6613

UniProt: P61956

Immunogen: Synthetic peptides of human SUMO2/3

Cross-Reactivity: Human, Mouse (Murine)

Description: Covalent attachment of one protein to another is one of the more prominentposttranslational modifications in respect to size and ubiquity. Ubiquitin is the mostfamiliar of the protein modifiers and its activation and transfer to target proteins has beenstudied extensively. Recently, a new group of ubiquitin-like proteins has been receiving alot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicatedin the stabilization of target proteins and/or their localization to sub-cellular complexes.

Characteristics: Synonyms: SUMO-2, SMT3B, SMT3H2, HSMT3, Sentrin-2, SUMO-3, SMT3 homolog 1, SMT3H1, Smallubiquitin-like modifier

Application Details

Application Notes: Western blot: 1-3 µg/ml. Recommended Positive Control: HL60 or NIH 3T3. A 15 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin, 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Proteases, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of SUMO2/3 in 15 ugs of HL60 cell lysate using ABIN123455 at 1 ug/ml.

Publications

Kahyo, Nishida, Yasuda: "Involvement of PIAS1 in the sumoylation of tumor suppressor p53." in: Molecular cell, Vol. 8, Issue 3, pp. 713-8, 2001 (PubMed).