Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B)

Synonyms: CVID, TACI, CD267, FLJ39942, MGC39952, MGC133214, TNFRSF14B, TNFRSF13B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN123458

Additional Information

Alternative name: CD267 / TACI

UniProt: O14836

Immunogen: Synthetic peptide corresponding to amino acids 116-132 of human TACI.

Cross-Reactivity: Human, Mouse (Murine)

Description: Simultaneously four different laboratories identified a a new member of the tumor necrosis factor (TNF) family. This has been named as TALL-1 (1), THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase (2), BAFF (for B cell activating factor belonging to the TNF family) (3) and BlyS (B lymphocyte stimulator) (4). Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases. The receptor for BlyS/BAFF appears to be expressed predominantly on B cells and activated T cells (5). Recently two orphan receptors TACI (6) and BCMA (,8) have been shown to be receptors for BlyS/BAFF (6-9). TACI is expressed on B cells and signals through CAML, activating the transcription factors NF-AT, NF-kB, and AP-1. TACI is a type III transmembrane protein, as defined by the lack of a cleaved signal sequence and its N-terminal extracellular exposure. The cDNA codes for 293 amino acids with predicted molecular weight of approximately 32 kDa.

Specificity: This antibody is specific for TACI. Human and mouse TACI amino acid sequences are 65% conserved in the immunogen

Application Details

Application Notes: Western blot: 0.1-0.5 ug/ml. Human spleen cells, Raw cells, or Daudi cells can be used as positive control. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 0.1 ug/ml of TACI in 10 ug/lane of Daudi lysate. A protein band of approximately 32 kD is detected.

Publications

Laabi, Gras, Carbonnel et al.: "A new gene, BCM, on chromosome 16 is fused to the interleukin 2 gene by a t(4;16)(q26;p13) translocation in a malignant T cell lymphoma." in: The EMBO journal, Vol. 11, Issue 11, pp. 3897-904, 1992 (PubMed).

Gras, Laabi, Linares-Cruz et al.: "BCMAp: an integral membrane protein in the Golgi apparatus of human mature B lymphocytes." in: International immunology, Vol. 7, Issue 7, pp. 1093-106, 1996 (PubMed).

Shu, Hu, Johnson: "TALL-1 is a novel member of the TNF family that is down-regulated by mitogens." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 680-3, 1999 (PubMed).

Mukhopadhyay, Ni, Zhai et al.: "Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 15978-81, 1999 (PubMed).

Schneider, MacKay, Steiner et al.: "BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1747-56, 1999 (PubMed).

Moore, Belvedere, Orr et al.: "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 260-3, 1999 (PubMed).

Marsters, Yan, Pitti et al.: "Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI." in: Current biology : CB, Vol. 10, Issue 13, pp. 785-8, 2000 (PubMed).