Toll-Like Receptor 4 (TLR4) antibody

Antigen: Toll-Like Receptor 4 (TLR4)

Synonyms: TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123464

Additional Information

Alternative name: CD284 / TLR4

UniProt: O00206

Immunogen: Portion of amino acids 100-150 of human TLR4

Cross-Reactivity: Human, Mouse (Murine)

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).

Application Details

Application Notes: Western blot (2-5 ug/ml, Observed size: ~90 kD (native) or 80 kD (partial recombinant mouse TLR4--extracellular portion plus His-tag). Flow Cytometry (2 - 4 ug/ 1x10e6 cells, intracellular). Immonoprecipitation: Please see Ueta et (2004) for details. Recommended Positive Control: Intestine cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of TLR4 using ABIN123464 at 2 ug/ml on (A) 0.1 ug /lane recomb. mouse TLR4 protein, (B) 20 ug/lane hum. intestine, and (C) 20 ug/lane mouse intestine.

Flow cytometric analysis of TLR4 in perme-abilized Ramos cells using ABIN123464 at 4 ug / 106 cells (open histogram).

Publications

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Poltorak, Ricciardi-Castagnoli, Citterio et al.: "Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2163-7, 2000 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Ueta, Nochi, Jang et al.: "Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 5, pp. 3337-47, 2004 (PubMed).