Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN123465

Toll-Like Receptor 4 (TLR4) (AA 418-435), (AA 420-435) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Epitope
»Alternatives AA 418-435, AA 420-435
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 5 references available
Catalog no. ABIN123465
Quantity 0.1 mg
Price
445.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to amino acids 418-435 of mouse TLR4
Purification Protein G Chromatography.
Alternative Name CD284 / TLR4
Background The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa (6). Alternate Names: TLR-4, hToll, Toll-like Receptor 4.
UniProt Q9QUK6
Research Area Signaling, Immunology, Inflammation
Application Notes Western blot analysis (1-3 ug/ml). This antibody was tested against partial recombinant mouse TLR4 (extracellular portion plus His-tag), and a 75-80 kDa band was observed. Immunoyctochemistry, Immunohistochemistry on paraffin section (1:50). ICC/IHC analysis has been performed by collaborator to validate usage in cell lines and human tissue samples. At this time data appears to be encouraging, but has yet to be duplicated and/or optimized. Testing is on-going, but researchers are encouraged to optimize. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.5% BSA as stabilizer and 0.05% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 2-8°C, for up to 6 months. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Shelf Life: One year from despatch. General.
Expiry Date 12 months
Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Poltorak, Ricciardi-Castagnoli, Citterio et al.: "Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2163-7, 2000 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts (141), (126), (20), (5)
Reactivities (222), (88), (35), (25), (24), (21), (19), (12), (4), (3), (2), (2), (1), (1), (1)
Applications (150), (141), (64), (62), (42), (32), (29), (18), (17), (10), (8), (7), (6), (3), (2), (1), (1), (1), (1)
Conjugates (30), (28), (18), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (23), (13), (9), (9), (7), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon