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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
TNF Receptor-Associated Factor 6 (TRAF6) antibody
Human, Mouse (Murine)
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml) (Variants)|
|Price||319.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||TRAF6 / RNF85|
|Immunogen||Synthetic peptide corresponding to amino acids 436-449 of human TRAF6|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6 (5). Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function (6). Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone Western blot under reducing conditions (3 µg/lane Apolipoprotein E), this antibody gave a major band at ~30 kDa when used at 1/500-1/1000., LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation (7). Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation.|
|Synonyms||RNF85, MGC:3310, AI851288, 2310003F17Rik, C630032O20Rik, LOC716907, xtraf6, traf6-B, MGC81955, DTRAF2, TRAF, TRAF2, TRAF6, dTRAF6, DmelCG10961, CG10961, rnf85, MGC79673, MGC128039, GB10539, fi15f02, MGC63704, zgc:63704, wu:fi15f02, traf6|
|Application Notes||Western blot (1:500-1:1000, a ~54 kDa band is detected). Recommended Positive Control: Human thymus, testis cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.05% BSA and 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of TRAF6 using ABIN123472 at 1:1000 dilution against 15 mg of (A) Jurkat cell lysate and (B) NIH3T3 cell lysate.|
Cheng, Cleary, Ye et al.: "Involvement of CRAF1, a relative of TRAF, in CD40 signaling." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1494-8, 1995 (PubMed).
Rothe, Wong, Henzel et al.: "A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor." in: Cell, Vol. 78, Issue 4, pp. 681-92, 1994 (PubMed).
Cao, Xiong, Takeuchi et al.: "TRAF6 is a signal transducer for interleukin-1." in: Nature, Vol. 383, Issue 6599, pp. 443-6, 1996 (PubMed).
Lomaga, Yeh, Sarosi et al.: "TRAF6 deficiency results in osteopetrosis and defective interleukin-1, CD40, and LPS signaling." in: Genes & development, Vol. 13, Issue 8, pp. 1015-24, 1999 (PubMed).
Ding, Wong, Chow et al.: "Quantitative subtyping of hepatitis B virus reveals complex dynamics of YMDD motif mutants development during long-term lamivudine therapy." in: Antiviral therapy, Vol. 11, Issue 8, pp. 1041-9, 2007 (PubMed).
|Hosts||Rabbit (67), Mouse (1)|
|Reactivities||Human (61), Rat (Rattus) (42), Mouse (Murine) (40), Cow (Bovine) (2), Dog (Canine) (1), Pig (Porcine) (1)|
|Applications||Western Blotting (WB) (49), Immunofluorescence (IF) (26), ELISA (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunohistochemistry (IHC) (12), Immunocytochemistry (ICC) (11), Immunoprecipitation (IP) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Biotin (2), FITC (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (9), Internal Region (5), AA 451-469 (3), AA 436-449 (1), Internal Region,AA 314-343 (1)|