TNFRSF19 / TRADE antibody

Antigen: TNFRSF19 / TRADE

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN123477

Additional Information

Alternative name:

UniProt: Q9NS68

Immunogen: Mixture of synthetic peptides corresponding to amino acids 29-44, 204-219, and 409-421 of human TROY

Cross-Reactivity: Human, Mouse (Murine)

Description: Analysis of EST clones and use of a signal sequence trap screening of the murine brain have lead to identification of a new member of the tumor necrosis factor receptor superfamily designated as TAJ and TROY, respectively (2,3). TAJ/TROY is a membrane protein of 423 amino acids with characteristic cysteine-rich motifs in the extracellular domain and a tumor necrosis factor receptor-associated factor (TRAF) 2 binding sequence in the cytoplasmic domain. Human TROY has 78% identity at the amino acid level with mouse TROY. The extracellular domain of TROY has significant homology with that of EDAR (another newly identified receptor belonging to TNF receptor family) that specifies hair follicle fate (1). The cytoplasmic domain of TAJ is different than other TNF receptor family members. Although its cytoplasmic domain does not contain death domain, TAJ/TROY induced cell death is probably mediated through a caspase- independent pathway. TAJ-induced JNK activation was not blocked by dominant-negative inhibitors of TRAF2, TRAF5, or ASK1, which have been previously implicated in JNK activation via TNFR1 and CD40. However, coimmunoprecipitation assays revealed that TAJ is capable of binding a number of different TRAF family members, and it is possible that TAJ-induced JNKactivation is mediated by an as yet untested TRAF homolog, such as TRAF6. TROY mRNA is highly expressed in brain, prostate and embryo and moderately expressed in the heart, lung, liver.

Application Details

Application Notes: Western blot: 0.5 - 2.0 ug/ml. Recommended positive control: T98G. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of TAJ/TROY using ABIN123477 in T98G, a human glioblastoma cell line lysate. A protein band of approximately 46 kD is detected.

Publications

Monreal, Ferguson, Headon et al.: "Mutations in the human homologue of mouse dl cause autosomal recessive and dominant hypohidrotic ectodermal dysplasia." in: Nature genetics, Vol. 22, Issue 4, pp. 366-9, 1999 (PubMed).

Kojima, Morikawa, Copeland et al.: "TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles." in: The Journal of biological chemistry, Vol. 275, Issue 27, pp. 20742-7, 2000 (PubMed).