Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)

Synonyms: FN14, CD266, TWEAKR

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123478

Additional Information

Alternative name: CD266 / TweakR

UniProt: Q9NP84

Immunogen: Synthetic peptide containing amino acids 104-118 of human TweakR

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines (1, 2). Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library (3). TweakR is a small protein belonging to TNF receptor super family. TweakR is capable of intiating a proliferative signal by biding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in aorta and liver. It may also play a role in vascular cell migration and angiogenesis. Alternate Names: TNFRSF12A, FN14, TWEAK-Receptor, FGF-Inducible 14, Tumor Necrosis Factor Receptor Superfamily Member 12A, Fibroblast Growth Factor Inducible Immediate Early Response Protein 14.

Application Details

Application Notes: Western blot: 2-4 ug/ml. Recommended Positive Control: human, mouse, rat liver. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Receptors

Restrictions: For Research Use only

Images

Western blot analysis of TweakR in 293 cells. Untransfected (lane 1) or transfectd with human TweakR (lane 2). Photograph courtesy of Dr. Taly Spivakk, The MD Anderson Cancer Center,Houston,TX.

Western blot analysis of TweakR in A) human liver tissue lysate and B) mouse liver tissue lysate using ABIN123478at 1:500.

Publications

Chicheportiche, Bourdon, Xu et al.: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, 1998 (PubMed).

Chicheportiche, Fossati-Jimack, Moll et al.: "Down-regulated expression of TWEAK mRNA in acute and chronic inflammatory pathologies." in: Biochemical and biophysical research communications, Vol. 279, Issue 1, pp. 162-5, 2000 (PubMed).

Wiley, Cassiano, Lofton et al.: "A novel TNF receptor family member binds TWEAK and is implicated in angiogenesis." in: Immunity, Vol. 15, Issue 5, pp. 837-46, 2001 (PubMed).