Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) (AA 12-28) antibody

Details for Product No. ABIN123480
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AA 12-28
(15), (14), (13), (2), (2), (1), (1), (1)
(67), (20), (20), (1)
(55), (11), (1)
(6), (6), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(57), (33), (10), (5), (4), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Immunogen A mixture of synthetic peptides containing amino acids 12-28 of human UBE2V2
Purification Protein G Chromatography
Alternative Name UBE2V2 (UBE2V2 Antibody Abstract)
Background The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3 (1). This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family (2). This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18- dependent postreplicative DNA repair in yeast (3). These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair (6,7). Alternate Names: Mms2.
UniProt Q15819
Application Notes Western blot: 2.0 ug/ml. Recommended positive control: HL-60. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) (AA 12-28) antibody (ABIN123480) Detection of UBE2V2 inHL-60 cell lysate with anti-UBE2V2 pcAb (ABIN123480) at 2ug/ml ...
General Michelin, Ruller, Ward et al.: "Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii." in: Journal of industrial microbiology & biotechnology, Vol. 35, Issue 1, pp. 17-25, 2007 (PubMed).

VanDemark, Hofmann, Tsui et al.: "Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer." in: Cell, Vol. 105, Issue 6, pp. 711-20, 2001 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Ulrich, Jentsch: "Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair." in: The EMBO journal, Vol. 19, Issue 13, pp. 3388-97, 2000 (PubMed).

Broomfield, Chow, Xiao: "MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 10, pp. 5678-83, 1998 (PubMed).

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Catalog No. ABIN123480
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