Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2)

Synonyms: MMS2, UEV2, EDPF1, UEV-2, DDVIT1, EDAF-1, EDPF-1, DDVit-1, C81524, AI848315, MGC102399, 6820402M05, 1110021H13Rik, 4632410D19Rik, 5730524P06Rik, TMEM189-UBE2V1, UBE2V2, MGC128699, MGC105268, ube2v2, MGC53645, MGC130807, UBE2V1, CROC-1B, zgc:63717, wu:fb18b11, wu:fb24d11, mms2, uev2, edpf1, uev-2, ddvit1, edaf-1, edpf-1, ddvit-1, DKFZp459H067

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN123480

Additional Information

Alternative name: UBE2V2

UniProt: Q15819

Immunogen: A mixture of synthetic peptides containing amino acids 12-28 of human UBE2V2

Cross-Reactivity: Human

Description: The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3 (1). This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family (2). This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18- dependent postreplicative DNA repair in yeast (3). These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair (6,7). Alternate Names: Mms2.

Synonyms: MMS2, UEV2, EDPF1, UEV-2, DDVIT1, EDAF-1, EDPF-1, DDVit-1, C81524, AI848315, MGC102399, 6820402M05, 1110021H13Rik, 4632410D19Rik, 5730524P06Rik, TMEM189-UBE2V1, UBE2V2, MGC128699, MGC105268, ube2v2, MGC53645, MGC130807, UBE2V1, CROC-1B, zgc:63717, wu:fb18b11, wu:fb24d11, mms2, uev2, edpf1, uev-2, ddvit1, edaf-1, edpf-1, ddvit-1, DKFZp459H067

Application Details

Application Notes: Western blot: 2.0 ug/ml. Recommended positive control: HL-60. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Detection of UBE2V2 inHL-60 cell lysate with anti-UBE2V2 pcAb (ABIN123480) at 2ug/ml dilution.

Publications

Broomfield, Chow, Xiao: "MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 10, pp. 5678-83, 1998 (PubMed).

Ulrich, Jentsch: "Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair." in: The EMBO journal, Vol. 19, Issue 13, pp. 3388-97, 2000 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

VanDemark, Hofmann, Tsui et al.: "Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer." in: Cell, Vol. 105, Issue 6, pp. 711-20, 2001 (PubMed).

Michelin, Ruller, Ward et al.: "Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii." in: Journal of industrial microbiology & biotechnology, Vol. 35, Issue 1, pp. 17-25, 2007 (PubMed).