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Ubiquitin-Conjugating Enzyme E2 Variant 2 (UBE2V2) antibody
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||308.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Immunogen||A mixture of synthetic peptides containing amino acids 12-28 of human UBE2V2|
|Description||The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3 (1). This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family (2). This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18- dependent postreplicative DNA repair in yeast (3). These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair (6,7). Alternate Names: Mms2.|
|Synonyms||MMS2, UEV2, EDPF1, UEV-2, DDVIT1, EDAF-1, EDPF-1, DDVit-1, C81524, AI848315, MGC102399, 6820402M05, 1110021H13Rik, 4632410D19Rik, 5730524P06Rik, TMEM189-UBE2V1, UBE2V2, MGC128699, MGC105268, ube2v2, MGC53645, MGC130807, UBE2V1, CROC-1B, zgc:63717, wu:fb18b11, wu:fb24d11, mms2, uev2, edpf1, uev-2, ddvit1, edaf-1, edpf-1, ddvit-1, DKFZp459H067|
|Application Notes||Western blot: 2.0 ug/ml. Recommended positive control: HL-60. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2% gelatin and 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Detection of UBE2V2 inHL-60 cell lysate with anti-UBE2V2 pcAb (ABIN123480) at 2ug/ml dilution.|
Broomfield, Chow, Xiao: "MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 10, pp. 5678-83, 1998 (PubMed).
Ulrich, Jentsch: "Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair." in: The EMBO journal, Vol. 19, Issue 13, pp. 3388-97, 2000 (PubMed).
Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).
VanDemark, Hofmann, Tsui et al.: "Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer." in: Cell, Vol. 105, Issue 6, pp. 711-20, 2001 (PubMed).
Michelin, Ruller, Ward et al.: "Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii." in: Journal of industrial microbiology & biotechnology, Vol. 35, Issue 1, pp. 17-25, 2007 (PubMed).
|Hosts||Rabbit (35), Goat (2), Mouse (1)|
|Reactivities||Human (37), Mouse (Murine) (22), Rat (Rattus) (22), Cow (Bovine) (19), Chicken (18), Pig (Porcine) (18), Rabbit (18)|
|Applications||Western Blotting (WB) (22), Immunofluorescence (IF) (17), ELISA (15), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||N-Term (6), C-Term (4), AA 12-28 (1), Internal Region,AA 74-104 (1)|