Uracil-DNA Glycosylase (UNG) antibody

Antigen: Uracil-DNA Glycosylase (UNG)

Synonyms: MGC108922, DGU, UDG, UNG1, UNG2, HIGM4, UNG15, DKFZp781L1143, UNG, MGC140717, MGC56102, zgc:56102, ECK2578, JW2564, SPS, MGC82673

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Chimpanzee, Chicken, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123481

Additional Information

UniProt: P13051

Immunogen: A synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1).

Cross-Reactivity: Dog (Canine), Chimpanzee, Chicken, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis

Description: The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil- DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB (2, 3) The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis (5). Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type (5). Additionally, the expression of UNG is cell cycle regulated (Nagelhus et al. 1995). The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells (reviewed in Kruman et al. 2004). UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.

Application Details

Application Notes: Western blot (2 ug/ml). HeLa cell lysate can be used as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: The specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: This antibody is supplied as liquid, protein G affinity purified Ig fraction in PBS pH 7.2, containing 0.2% gelatine as stabilizer and 0.05% sodium azide as preservative.

Storage: Stable at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Detection of UNG in HeLa cell lysate with anti-UNG pcAb at 2 ug/ml dilution.

Publications

Slupphaug, Markussen, Olsen et al.: "Nuclear and mitochondrial forms of human uracil-DNA glycosylase are encoded by the same gene." in: Nucleic acids research, Vol. 21, Issue 11, pp. 2579-84, 1993 (PubMed).

Nilsen, Otterlei, Haug et al.: "Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene." in: Nucleic acids research, Vol. 25, Issue 4, pp. 750-5, 1997 (PubMed).

Bharati, Krokan, Kristiansen et al.: "Human mitochondrial uracil-DNA glycosylase preform (UNG1) is processed to two forms one of which is resistant to inhibition by AP sites." in: Nucleic acids research, Vol. 26, Issue 21, pp. 4953-9, 1998 (PubMed).

Nilsen, Rosewell, Robins et al.: "Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication." in: Molecular cell, Vol. 5, Issue 6, pp. 1059-65, 2000 (PubMed).

Krokan, Otterlei, Nilsen et al.: "Properties and functions of human uracil-DNA glycosylase from the UNG gene." in: Progress in nucleic acid research and molecular biology, Vol. 68, pp. 365-86, 2001 (PubMed).