Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) antibody

Antigen: Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)

Synonyms: MCH2, Mch2, mCASP-6, MGC93335, xCaspase-6, mch2, caspase-6, CASP6, MGC127633, zgc:112960, casp6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN123491

Additional Information

Alternative name: Caspase-6

UniProt: P55212

Immunogen: A synthetic peptide sequence corresponding to amino acids 250-264 of human caspase-6

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-6 encodes a 34-kD protein that is highly homologous to Caspase-1, -2 and -3. Two transcripts of 1.7-kb (Mch2a/Caspase6) and 1.4-kb (Mch2b) transcripts are expressed in Jurkat lymphocytes and other cell lines possibly due to alternate splicing.

Application Details

Application Notes: Western blot: 2.0 - 4.0 ug/ml. This antibody recognizes both the 34 kD alpha isoform and the 22 kD beta isoform of caspase-6. Recommended Positive Control: rat brain or Jurkat. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Affinity Purified

Buffer: BBS

Storage: Store the antibody at 2-8 °C for one month or at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis for human Caspase-6 using Jurkat cell lysate (lane A) or rat brain cell lysate (lane B) with ABIN123491 at 2 ug/ml. This antibody detects both 34 kDa (lane A) and 22 kDa (lane B) caspase-6 corresponding to the alpha and beta isoforms, respectively. Due to expression level, both isoforms may not be present in each sample tested.

Publications

Fernandes-Alnemri, Litwack, Alnemri: "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family." in: Cancer research, Vol. 55, Issue 13, pp. 2737-42, 1995 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Mitamura, Ikawa, Mizuno et al.: "Cytosolic nuclease activated by caspase-3 and inhibited by DFF-45." in: Biochemical and biophysical research communications, Vol. 243, Issue 2, pp. 480-4, 1998 (PubMed).