Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1) antibody

Antigen: Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1)

Synonyms: ICE, P45, IL1BC, Il1bc, Ice, DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370, CASP1, LOC100033888

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN123499

Additional Information

Alternative name: Caspase-1

UniProt: P29466

Immunogen: A synthetic peptide corresponding to amino acids 31-45 (QTRVLNKEEMEKVKR) of human caspase-1

Cross-Reactivity: Human, Mouse (Murine)

Application Details

Application Notes: Western blot (0.5 - 2 ug/ml) and immunohistochemistry on paraffim embeddeed sections. Optimal dilutions of this antibody are dependent on application and should be determined by the user. Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for caspase-1 expression using SP7344 with a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed Western blot analysis of caspase-1 in HeLa cell lysate using ABIN123499 at 0.5 ug/ml.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: This antibody is supplied as Protein G purified IgG PBS containing 0.2% gelatin and 0.05% sodium azide.

Storage: Store the antibody at 4°C for one month or in aliquots at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of caspase-1 in HeLa cell lysate using ABIN123499 at 0.5 ug/ml.

Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for caspase-1 expression using SP7344 with a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed

Publications

Cerretti, Kozlosky, Mosley et al.: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, 1992 (PubMed).

Li, Allen, Banerjee et al.: "Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock." in: Cell, Vol. 80, Issue 3, pp. 401-11, 1995 (PubMed).

Yuan, Shaham, Ledoux et al.: "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme." in: Cell, Vol. 75, Issue 4, pp. 641-52, 1993 (PubMed).

Tatsuta, Shiraishi, Mountz: "The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14248-54, 2000 (PubMed).