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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
APIP (APIP) antibody
|Synonyms||APIP2, CGI29, CGI-29, MMRP19, dJ179L10.2|
Alternatives Western Blotting (WB)
|4 references available|
|Quantity||0.1 mg (0.5 mg/ml) (Variants)|
|Price||297.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Immunogen||A full-length His-tagged recombinant APIP protein|
|Description||The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N- terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney. Alternate Names: CG129 / MMRP19.|
|Application Notes||Western blot (1-2 ug/ml). Recommended Positive Control: human muscle. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2% gelatin and 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of APIP2 in human muscle using ABIN123500 at 2 ug/ml.|
Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).
Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).
Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).
Nguyen, Lee, Cho et al.: "Crystallization and Preliminary X-Ray Studies of TON_0559, a Putative Member of the Haloacid Dehalogenase (HAD) Superfamily from Thermococcus onnurineus NA1." in: Protein and peptide letters, Vol. 15, Issue 2, pp. 235-7, 2008 (PubMed).
|Hosts||Rabbit (30), Mouse (6)|
|Reactivities||Human (35), Mouse (Murine) (22), Rat (Rattus) (22), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (17), Pig (Porcine) (17), Sheep (Ovine) (17), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (19), Immunofluorescence (IF) (15), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||AA 127-140 (1), AA 13-30 (1), Internal Region (1)|