Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ) antibody

Antigen: Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, zeta (NFKBIZ)

Synonyms: IKBZ, INAP, MAIL, FLJ30225, FLJ34463, Mail, AA408868, NFKBIZ, RGD1310834

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Monkey, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN123508

Additional Information

Alternative name: NFKBIZ / IKBZ

Gene ID: 64332

UniProt: Q9BYH8

Immunogen: Synthetic peptides corresponding to amino acids 684-699 and 285-298 of Mouse IkBzproteinAA Sequence: RKGADPSTRNLENEQP (684-699), YQYSPQTQNMQQPP (258-298)

Cross-Reactivity: Cow (Bovine), Human, Monkey, Mouse (Murine), Rat (Rattus)

Description: IkappaBzeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclearprotein that is highly homologous to the IkappaB family member Bcl-3. Transcription ofIkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in culturedB-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes,fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with theNF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit theDNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.

Characteristics: Synonyms: I-kappa-B-zeta, IkB-zeta, IkappaBzeta, INAP, MAIL, NF-kappa-B inhibitor zeta, Moleculepossessing ankyrin repeats induced by lipopolysaccharide, IL-1 inducible nuclearankyrin-repeat protein

Application Details

Application Notes: Western Blot: 1-3 µg/ml. Recommended Positive Control: RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS, containing 0.2% Gelatin as stabilizer and 0.05% Sodium Azide aspreservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of IkappaBz using antibody ABIN123508 at 3 ug/ml in mouse RAW cell lysate.

Publications

Yamazaki, Muta, Takeshige: "A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27657-62, 2001 (PubMed).

Kitamura, Matsushita, Iwanaga et al.: "Bacterial lipopolysaccharide-induced expression of the IkappaB protein MAIL in B-lymphocytes and macrophages." in: Archives of histology and cytology, Vol. 66, Issue 1, pp. 53-62, 2003 (PubMed).

Ito, Okada, Mochida et al.: "Molecular cloning of bullfrog corticotropin-releasing factor (CRF): effect of homologous CRF on the release of TSH from pituitary cells in vitro." in: General and comparative endocrinology, Vol. 138, Issue 3, pp. 218-27, 2004 (PubMed).