CD207 Molecule, Langerin (CD207) antibody
| Antigen |
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| Synonyms |
CD207, CLEC4K |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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PE (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN123512 |
| Quantity |
0.1 mg (0.5 mg/ml) |
| Price |
324.50 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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CD207 / Langerin
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UniProt
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Q8VBX4
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Immunogen
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Amino acids 36-44 of mouse Langerin
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Cross-Reactivity
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Mouse (Murine), Rat (Rattus)
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Isotype
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IgG
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Description
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Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Lamgerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation. Alternate Names: CD207.
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Specificity
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This antibody is specific for Langerin. Genbank Accession No. CAC82936
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Application Notes
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Western Blot: 1 - 3 ug/ml. A protein band of ~36 kDa is detected in mouse lung tissue lysate. Pre-incubation with the immunizing peptide completely removes langerin reactivity from ABIN123512. IHC (FFPE): 2.5-5 ug/ml. Flow (Intracellular): see Ness et al for details. Recommended positive control: Mouse lung tissue lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Purification
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Purified
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Buffer
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PBS containing 0.05% BSA and 0.05% sodium azide
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Storage
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Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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CD Antigens
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Restrictions
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For Research Use only
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Valladeau, Ravel, Dezutter-Dambuyant et al.: "Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules." in: Immunity, Vol. 12, Issue 1, pp. 71-81, 2000 (PubMed).
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Alternatives for antigen "CD207 Molecule, Langerin (CD207)", type "Antibodies"
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Hosts
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Rabbit (26), Mouse (3)
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Reactivities
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Human (26), Mouse (Murine) (21), Rat (Rattus) (20), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
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Applications
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Flow Cytometry (FACS) (17), Immunofluorescence (IF) (16), Western Blotting (WB) (11), ELISA (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
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Conjugates
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PE (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (2), AA 36-44 (1), AA 64-328 (1)
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2013 BioMed Central Research Awards
