Aminolevulinate, delta-, Synthase 1 (ALAS2) antibody

Antigen: Aminolevulinate, delta-, Synthase 1 (ALAS2)

Synonyms: ALAS, MIG4, ALAS3, ALASH, ALAS-N, Alas-1, Alas-h, ALAS1, alas1, MGC79710, ALAS-H, ALASN, DKFZp469K1012, ASB, ANH1, XLSA, ALASE, ALAS-E, FLJ93603, Alas-2, sau, alas-e, cb1063, sauternes, MGC82115, asb, anh1, xlsa, MGC89126, ALAS2, MGC128330, DKFZp459M1818, MGC81838

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis

Application: Western Blotting (WB)

Catalog no.: ABIN123539

Additional Information

Alternative name: ALAS1

Swiss-Prot: P13196

Immunogen: A portion of amino acids 480-530 of human ALAS-1

Description: ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme Western blot under reducing conditions (3 µg/lane Apolipoprotein E), this antibody gave a major band at ~30 kDa when used at 1/500-1/1000. and liver function. Alternate Names: Migration inducing protein 4 (MIG4).

Specificity: This antibody is specific for ALAS-1. The amino acid sequence used as immunogen is 100% homologous in human, chimp and beluga whale, 94% in dog and chicken, 88% in mouse, rat and lungfish, 83% in bovine, xenopus, pufferfish, 77% in coelacanth. Genebank Info (Protein): P13196 Genebank Info (Nucleotide): NP_000679, NP_954635

Synonyms: ALAS, MIG4, ALAS3, ALASH, ALAS-N, Alas-1, Alas-h, ALAS1, alas1, MGC79710, ALAS-H, ALASN, DKFZp469K1012, ASB, ANH1, XLSA, ALASE, ALAS-E, FLJ93603, Alas-2, sau, alas-e, cb1063, sauternes, MGC82115, asb, anh1, xlsa, MGC89126, ALAS2, MGC128330, DKFZp459M1818, MGC81838

Application Details

Application Notes: Western blot (1-3 ug/ml). Recommended positive control: HepG2 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human)

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of ALAS-1 in HepG2 cell lysate using antibody ABIN123539 at 1 ug/ml.