Toll-Like Receptor 10 (TLR10) antibody

Antigen: Toll-Like Receptor 10 (TLR10)

Synonyms: CD290, MGC104967, MGC126398, MGC126399, TLR10, MGC140623

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN123540

Additional Information

Alternative name: CD290 / TLR10

UniProt: Q9BXR5

Immunogen: Synthetic peptides corresponding to amino acids 37-52, 235-251, and 392-409 of TLR10 AA Sequence:MSLRKVPADLTPATTT / RNLSLENAKTSVLLLNK / FANNTPLEHLDLSQNLL

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Description: Toll-like receptors (TLRs) are one of the mechanisms by which the innate immune system senses the invasion of pathogenic microorganisms (reviewed in 1). TLRs are primary transmembrane proteins of immune cells, they have an extracellular region which contains leucine-rich repeat motifs, and a cytoplasmic tail, which has a Toll/interleukin-1 (IL-1) receptor (TIR) domain. TLRs are pattern recognition receptors (PRRs ) that bind to pathogen-associated molecular patterns (PAMPs). Different TLRs recognize different surface and intracellular components of microorganisms or PAMPS. The interaction between a TLR and a microbial component or PAMP triggers the activation of the innate immune system, as well as the development of acquired immunity. Interaction of PAMPs with different TLRs induces distinct patters of gene expression through signal transduction pathways. TLR signaling occurs through the TIR domains which utilize adaptors such as MyD88, TIRAP, TRIF, and TRAM. NF-kapppa-B and JNK pathways are activated leading to the transcription of genes encoding pro- inflammatory cytokines. TLR-10, originally identified from a spleen cDNA library, is on chromosome 4p14 and has been found to be expressed in lung and in B-lymphocytes (2). TLR10 is thought to be a potential asthma candidate gene because early life innate immune responses to ubiquitous inhaled allergens and PAMPs may influence asthma susceptibility. Alternate Names: CD290.

Application Details

Application Notes: ELISA: 1:100-1:1000 (it strongly recognizes amino acids 37-52, and weakly recognizes amino acids 235-251 and 392-409). Western blot (5 ug/ml). Recommended Positive Control: mouse or rat intestine cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of TLR10 in A) human intestine, B) mouse intestine, C) rat intestine and D) rat intestine with blocking peptides, using ABIN123540 at 5 ug/ml

Publications

Yang, Wang, Yasuo et al.: "[Effects of global warming on productivity and soil moisture in Taihang mountain: a transplant study]" in: Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban, Vol. 13, Issue 6, pp. 667-71, 2002 (PubMed).

Lazarus, Raby, Lange et al.: "TOLL-like receptor 10 genetic variation is associated with asthma in two independent samples." in: American journal of respiratory and critical care medicine, Vol. 170, Issue 6, pp. 594-600, 2004 (PubMed).