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P55 Subunit of Human Transcription Factor II A antibody

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Western Blotting (WB)
Pubmed 3 references available
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Quantity 125 μL
Shipping to United States ( )
Availability Will be delivered in 5 to 6 Business Days
Specificity Alpha-TFIIA reacts with the p55 protein in HeLa nuclear extract by Western blotting.
Background The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (alpha-subunit), 19 kDa (beta-subunit) and 12 kDa (gamma-subunit). The alpha- and beta-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma-subunit), retains native TFIIA activity. alpha-TFIIA is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p55 subunit of the TFIIA complex.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
Background publications DeJong, Roeder: "A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA." in: Genes & development, Vol. 7, Issue 11, pp. 2220-34, 1993 (PubMed).

Buratowski, Hahn, Guarente et al.: "Five intermediate complexes in transcription initiation by RNA polymerase II." in: Cell, Vol. 56, Issue 4, pp. 549-61, 1989 (PubMed).

Ranish, Hahn: "The yeast general transcription factor TFIIA is composed of two polypeptide subunits." in: The Journal of biological chemistry, Vol. 266, Issue 29, pp. 19320-7, 1991 (PubMed).

Catalog No. ABIN123787
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