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P56 Subunit of Human Transcription Factor II E alpha antibody

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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Pubmed 4 references available
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Quantity 125 μL
Shipping to United States ( )
Availability Will be delivered in 5 to 6 Business Days
Specificity Alpha-TFIIE alpha reacts with the p56 protein in HeLa nuclear extract by Western blotting.
Alternative Name p56 subunit of human Transcription Factor IIEalpha
Background Consists of three subunits of 35 kDa (alpha-subunit), 19 kDa (beta-subunit) and 12 kDa (gamma-subunit). The alpha-and beta- subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma-subunit), retains native TFIIA activity. alpha-TFIIA is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p55 subunit of the TFIIA complex.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
Background publications Ohkuma, Sumimoto, Horikoshi et al.: "Factors involved in specific transcription by mammalian RNA polymerase II: purification and characterization of general transcription factor TFIIE." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 23, pp. 9163-7, 1991 (PubMed).

Flores, Ha, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF." in: The Journal of biological chemistry, Vol. 265, Issue 10, pp. 5629-34, 1990 (PubMed).

Inostroza, Flores, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE." in: The Journal of biological chemistry, Vol. 266, Issue 14, pp. 9304-8, 1991 (PubMed).

Peterson, Inostroza, Maxon et al.: "Structure and functional properties of human general transcription factor IIE." in: Nature, Vol. 354, Issue 6352, pp. 369-73, 1992 (PubMed).

Catalog No. ABIN123788
$428.51
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