P34 Subunit of Human Transcription Factor IIEbeta antibody

Details for Product No. ABIN123789
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN123789
Quantity 50 µg
Price
457.38 $   Plus shipping costs $45.00
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Specificity Alpha-TFIIE beta reacts with the p34 protein in HeLa nuclear extract by Western blotting.
Background The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (alpha) and 34 kDa (beta) subunits and is shown to be a heterotetramer. The 56 kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the preinitiation complex probably concomitant with RNA polymerase II and TFIIF. alpha-TFIIE beta is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p34 subunit of the TFIIE complex.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
General Peterson, Inostroza, Maxon et al.: "Structure and functional properties of human general transcription factor IIE." in: Nature, Vol. 354, Issue 6352, pp. 369-73, 1992 (PubMed).

Inostroza, Flores, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE." in: The Journal of biological chemistry, Vol. 266, Issue 14, pp. 9304-8, 1991 (PubMed).

Flores, Ha, Reinberg: "Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF." in: The Journal of biological chemistry, Vol. 265, Issue 10, pp. 5629-34, 1990 (PubMed).

Ohkuma, Sumimoto, Horikoshi et al.: "Factors involved in specific transcription by mammalian RNA polymerase II: purification and characterization of general transcription factor TFIIE." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 23, pp. 9163-7, 1991 (PubMed).

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