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P33 Subunit of RNA Polymerase II antibody

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Western Blotting (WB)
Pubmed 7 references available
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Quantity 50 μg
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Availability Will be delivered in 5 to 6 Business Days
Specificity Alpha-RNA Pol II (p33) reacts with the p33 protein in HeLa nuclear extract by Western blotting.
Background HRPB5 (p33) is a highl y conserved subunit shared by all three RNA pol ymerases. It has been shown to be in close contact to promoter DNA when Pol II is recruited into the preinitiation complex. RPB5 has also been implicated in direct protein-protein contacts with transcription factor IIB, RAP30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of Pol II, RMP (RPB5-mediating protein). Therefore, RPB5 is facilitating the communication between the Pol II core and a variety of basal and gene-specific transcription factors. In the Pol II complex, RPB5 interacts with RPB3 and the RPB3?RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. RPB5 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex. alpha-RNA Pol II (p33) is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p33 subunit of the polymerase complex.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
Background publications Wei, Dorjsuren, Lin et al.: "Direct interaction between the subunit RAP30 of transcription factor IIF (TFIIF) and RNA polymerase subunit 5, which contributes to the association between TFIIF and RNA polymerase II." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 12266-73, 2001 (PubMed).

Kimura, Ishihama: "Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II." in: Nucleic acids research, Vol. 28, Issue 4, pp. 952-9, 2000 (PubMed).

Dorjsuren, Lin, Wei et al.: "RMP, a novel RNA polymerase II subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein." in: Molecular and cellular biology, Vol. 18, Issue 12, pp. 7546-55, 1998 (PubMed).

Kim, Lagrange, Wang et al.: "Trajectory of DNA in the RNA polymerase II transcription preinitiation complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 23, pp. 12268-73, 1997 (PubMed).

Miyao, Yasui, Sakurai et al.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 1, Issue 9, pp. 843-54, 1997 (PubMed).

Lin, Nomura, Cheong et al.: "Hepatitis B virus X protein is a transcriptional modulator that communicates with transcription factor IIB and the RNA polymerase II subunit 5." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7132-9, 1997 (PubMed).

Woychik, Liao, Kolodziej et al.: "Subunits shared by eukaryotic nuclear RNA polymerases." in: Genes & development, Vol. 4, Issue 3, pp. 313-23, 1990 (PubMed).

Catalog No. ABIN123794
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