P14.5 Subunit of RNA Polymerase II antibody

Antigen: P14.5 Subunit of RNA Polymerase II

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN123795

Additional Information

Format: Liquid

Description: HRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and transcription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation. RNA polymerase II lacking the RPB9 subunit uses alternate transcription initiation sites in vitro and in vivo and is unable to respond to the transcription elongation factor TFIIS in vitro. A role in the modulation of initiation and elongation is consistent with the localization of RPB9 in the three-dimensional structure of yeast RNA polymerase II. RPB9 is located at the tip of the so-called 'jaws' of the enzyme, which is thought to function by clamping the DNA downstream of the active site. RPB9 comprises two zinc ribbon domains joined by a conserved linker region. The C-terminal zinc ribbon is similar in sequence to that found in TFIIS. alpha-RNA Pol II (p14.5) is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p14.5 subunit of the polymerase complex.

Specificity: Alpha-RNA Pol II (p14.5) reacts with the p14.5 subunit in HeLa nuclear extract by Western blotting.

Application Details

Application Notes: Recommended dilution range for Western blot analysis: 1:200 - 1:1000.

Storage: store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.

Restrictions: For Research Use only

Publications

Harrison, Mortin, Corces: "The RNA polymerase II 15-kilodalton subunit is essential for viability in Drosophila melanogaster." in: Molecular and cellular biology, Vol. 12, Issue 3, pp. 928-35, 1992 (PubMed).

Furter-Graves, Furter, Hall: "SHI, a new yeast gene affecting the spacing between TATA and transcription initiation sites." in: Molecular and cellular biology, Vol. 11, Issue 8, pp. 4121-7, 1991 (PubMed).

Woychik, Lane, Young: "Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes." in: The Journal of biological chemistry, Vol. 266, Issue 28, pp. 19053-5, 1991 (PubMed).

Qian, Jeon, Yoon et al.: "Structure of a new nucleic-acid-binding motif in eukaryotic transcriptional elongation factor TFIIS." in: Nature, Vol. 365, Issue 6443, pp. 277-9, 1993 (PubMed).

Nogi, Yano, Dodd et al.: "Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures." in: Molecular and cellular biology, Vol. 13, Issue 1, pp. 114-22, 1993 (PubMed).

Awrey, Weilbaecher, Hemming et al.: "Transcription elongation through DNA arrest sites. A multistep process involving both RNA polymerase II subunit RPB9 and TFIIS." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 14747-54, 1997 (PubMed).

Cramer, Bushnell, Fu et al.: "Architecture of RNA polymerase II and implications for the transcription mechanism." in: Science (New York, N.Y.), Vol. 288, Issue 5466, pp. 640-9, 2000 (PubMed).

Hemming, Jansma, Macgregor et al.: "RNA polymerase II subunit Rpb9 regulates transcription elongation in vivo." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35506-11, 2000 (PubMed).