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TATA-Binding Protein-Associated Phosphoprotein (DR1) antibody
Alternatives Western Blotting (WB)
|8 references available|
|Price||457.38 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 5 to 6 Business Days|
|Alternative name||Down-regulator of Transcription 1|
|Description||Dr1 is a general negative regulator of class II and class III gene expression. It binds to the basic repeat domain of TBP on promoter DNA and can prevent the RNA polymerase II holoenzyme, or its TFIIB and/or TFIIA subunits, from assembling into an initiation complex. Dr1 is phosphorylated in vivo and this modification affects its interaction with TBP. In addition, Dr1 interacts with the hyperphosphorylated form of Pol II and with the repression domain of the AREB6 repressor. Dr1 forms a heterodimer complex with DRAP1 through its histone fold domain. More recently it was discovered that Dr1-DRAP1 is a bi-functional basal transcription factor that differentially regulates gene transcription through DPE (downstream promoter elements) or TATA box motifs. It can stimulate transcription in vitro from Drosophila promoters containing DPEs, whereas it represses transcription from TATA-containing promoters. alpha-Dr1 is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length Dr1 protein.|
|Specificity||Alpha-Dr1 reacts with the Dr1 protein in HeLa nuclear extract by Western blotting.|
|Application Notes||Recommended dilution range for Western blot analysis: 1:200 - 1:1000.|
|Storage||store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.|
|Restrictions||For Research Use only|
Inostroza, Mermelstein, Ha et al.: "Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription." in: Cell, Vol. 70, Issue 3, pp. 477-89, 1992 (PubMed).
Kim, Zhao, Ge et al.: "TATA-binding protein residues implicated in a functional interplay between negative cofactor NC2 (Dr1) and general factors TFIIA and TFIIB." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10976-81, 1995 (PubMed).
White, Khoo, Inostroza et al.: "Differential regulation of RNA polymerases I, II, and III by the TBP-binding repressor Dr1." in: Science (New York, N.Y.), Vol. 266, Issue 5184, pp. 448-50, 1994 (PubMed).
Mermelstein, Yeung, Cao et al.: "Requirement of a corepressor for Dr1-mediated repression of transcription." in: Genes & development, Vol. 10, Issue 8, pp. 1033-48, 1996 (PubMed).
Goppelt, Stelzer, Lottspeich et al.: "A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains." in: The EMBO journal, Vol. 15, Issue 12, pp. 3105-16, 1996 (PubMed).
Ikeda, Halle, Stelzer et al.: "Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 10-8, 1998 (PubMed).
Castano, Gross, Wang et al.: "The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 13, pp. 7184-9, 2000 (PubMed).
Willy, Kobayashi, Kadonaga: "A basal transcription factor that activates or represses transcription." in: Science (New York, N.Y.), Vol. 290, Issue 5493, pp. 982-5, 2000 (PubMed).
|Reactivities||Human (26), Mouse (Murine) (25), Rat (Rattus) (23), Chicken (2), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Zebrafish (1)|
|Applications||Immunofluorescence (IF) (16), Flow Cytometry (FACS) (15), Western Blotting (WB) (12), ELISA (8), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Chromatin Immunoprecipitation (ChiP) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||Internal Region (1), Middle Region (1), N-Term (1)|