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TATA-Binding Protein-Associated Phosphoprotein (DR1) antibody

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Synonyms BG:DS00929.3, CG4185, Dmel\\CG4185, Dr1, NC2 beta, Nc2b, dDr1, dNC2, wu:fj15f04, zgc:152914, 1700121L09Rik, Dr1l, NC2, NC2beta, NC2-BETA
(43), (15), (9), (2), (1), (1), (1), (1)
(36), (7), (1)
(2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(40), (24), (7), (4), (1), (1), (1), (1)
Pubmed 8 references available
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Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 5 to 6 Business Days
Specificity Alpha-Dr1 reacts with the Dr1 protein in HeLa nuclear extract by Western blotting.
Alternative Name Down-regulator of Transcription 1 (DR1 Antibody Abstract)
Background Dr1 is a general negative regulator of class II and class III gene expression. It binds to the basic repeat domain of TBP on promoter DNA and can prevent the RNA polymerase II holoenzyme, or its TFIIB and/or TFIIA subunits, from assembling into an initiation complex. Dr1 is phosphorylated in vivo and this modification affects its interaction with TBP. In addition, Dr1 interacts with the hyperphosphorylated form of Pol II and with the repression domain of the AREB6 repressor. Dr1 forms a heterodimer complex with DRAP1 through its histone fold domain. More recently it was discovered that Dr1-DRAP1 is a bi-functional basal transcription factor that differentially regulates gene transcription through DPE (downstream promoter elements) or TATA box motifs. It can stimulate transcription in vitro from Drosophila promoters containing DPEs, whereas it represses transcription from TATA-containing promoters. alpha-Dr1 is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length Dr1 protein.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
Background publications Willy, Kobayashi, Kadonaga: "A basal transcription factor that activates or represses transcription." in: Science (New York, N.Y.), Vol. 290, Issue 5493, pp. 982-5, 2000 (PubMed).

Castano, Gross, Wang et al.: "The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 13, pp. 7184-9, 2000 (PubMed).

Ikeda, Halle, Stelzer et al.: "Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 10-8, 1998 (PubMed).

Goppelt, Stelzer, Lottspeich et al.: "A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains." in: The EMBO journal, Vol. 15, Issue 12, pp. 3105-16, 1996 (PubMed).

Mermelstein, Yeung, Cao et al.: "Requirement of a corepressor for Dr1-mediated repression of transcription." in: Genes & development, Vol. 10, Issue 8, pp. 1033-48, 1996 (PubMed).

White, Khoo, Inostroza et al.: "Differential regulation of RNA polymerases I, II, and III by the TBP-binding repressor Dr1." in: Science (New York, N.Y.), Vol. 266, Issue 5184, pp. 448-50, 1994 (PubMed).

Kim, Zhao, Ge et al.: "TATA-binding protein residues implicated in a functional interplay between negative cofactor NC2 (Dr1) and general factors TFIIA and TFIIB." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10976-81, 1995 (PubMed).

Inostroza, Mermelstein, Ha et al.: "Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription." in: Cell, Vol. 70, Issue 3, pp. 477-89, 1992 (PubMed).

Catalog No. ABIN123796
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