Did you know that you can buy products from over 140 different suppliers from us?

alpha-DNA Topoisomerase I antibody

Details for Product No. ABIN123798
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Pubmed 12 references available
Quantity 50 µg
Shipping to United States (Change)
Availability Will be delivered in 5 to 6 Business Days
Catalog No. ABIN123798
457.38 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity Alpha-Topo I reacts with the DNA Topoisomerase I protein in HeLa nuclear extract by Western blotting.
Background Human DNA Topoisomerase I (Topo I) is a monomeric protein of 765 anino acids encoded by a single-copy gene. Based on its function, DNA Topoisomerase I has been subdivided into four distinct domains. The N-terminal 214 amino acids of Topo I comprise a highly charged N-terminal domain involved in protein-protein interactions with a number of cellular proteins. The highly conserved core domain expanded from amino acids 215 to 636 contains most catalytic residues and retains DNA binding activity. The active C-terminal domain (residues 713-765) is connected to the core domain by a poorly conserved linker domain (residues 636-712). Human DNA Topoisomerase I is the best studied of the DNA Topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks and this kind of DNA damage closely relates to most cancer, it is therefore believed that DNA Topo I can be an important target for antitumor agents. alpha-Topo I is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length DNA Topoisomerase I protein.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
General Giovanella, Stehlin, Wall et al.: "DNA topoisomerase I--targeted chemotherapy of human colon cancer in xenografts." in: Science (New York, N.Y.), Vol. 246, Issue 4933, pp. 1046-8, 1990 (PubMed).

DArpa, Machlin, Ratrie et al.: "cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 8, pp. 2543-7, 1988 (PubMed).

Juan, Hwang, Liu et al.: "Human DNA topoisomerase I is encoded by a single-copy gene that maps to chromosome region 20q12-13.2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 23, pp. 8910-3, 1988 (PubMed).

Liu, Miller: "Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 6, pp. 3487-91, 1981 (PubMed).

Kretzschmar, Meisterernst, Roeder: "Identification of human DNA topoisomerase I as a cofactor for activator-dependent transcription by RNA polymerase II." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 24, pp. 11508-12, 1994 (PubMed).

Sekiguchi, Seeman, Shuman: "Resolution of Holliday junctions by eukaryotic DNA topoisomerase I." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 2, pp. 785-9, 1996 (PubMed).

Rossi, Labourier, Forne et al.: "Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I." in: Nature, Vol. 381, Issue 6577, pp. 80-2, 1996 (PubMed).

Stewart, Ireton, Parker et al.: "Biochemical and biophysical analyses of recombinant forms of human topoisomerase I." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7593-601, 1996 (PubMed).

Stewart, Ireton, Champoux: "The domain organization of human topoisomerase I." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7602-8, 1996 (PubMed).

Pourquier, Pilon, Kohlhagen et al.: "Trapping of mammalian topoisomerase I and recombinations induced by damaged DNA containing nicks or gaps. Importance of DNA end phosphorylation and camptothecin effects." in: The Journal of biological chemistry, Vol. 272, Issue 42, pp. 26441-7, 1997 (PubMed).

Redinbo, Stewart, Kuhn et al.: "Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA." in: Science (New York, N.Y.), Vol. 279, Issue 5356, pp. 1504-13, 1998 (PubMed).

Pommier, Pourquier, Fan et al.: "Mechanism of action of eukaryotic DNA topoisomerase I and drugs targeted to the enzyme." in: Biochimica et biophysica acta, Vol. 1400, Issue 1-3, pp. 83-105, 1998 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN123798
457.38 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top