Retinoid X Receptor, Ligand Binding Domain antibody

Details for Product No. ABIN123799
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Western Blotting (WB)
Pubmed 5 references available
Quantity 50 μg
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Specificity Alpha-RXR reacts with the RXR protein in HeLa nuclear extract by Western blotting.
Background Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain (LBD). RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, several constitutive active orphan nuclear receptors (e.g. nuclear growth factor I-B), oxysterol receptors, and constitutive androstane receptors. RXRs also form homodimers to mediate the effects of 9-cis-retinoic acid (9-cRA). Depending on these protein-protein interactions, RXR-containing complexes have distinct ligand-dependent and constitutive functions. The LBD is functionally complex and mediates ligand binding, receptor homo- and heterodimerization, repression of transcription in the absence of ligand, and ligand-dependent activation of transcription. Hormone binding to the structurally conserved LBD of the RXR triggers a conformational change that principally affects the conserved C-terminal transactivation helix H12 involved in transcriptional activation. alpha-RXR is an affinity-purified rabbit polyclonal antibody raised against the recombinant ligand-binding domain of the RXR protein.
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Restrictions For Research Use only
Format Liquid
Storage -80 °C
Background publications Egea, Rochel, Birck et al.: "Effects of ligand binding on the association properties and conformation in solution of retinoic acid receptors RXR and RAR." in: Journal of molecular biology, Vol. 307, Issue 2, pp. 557-76, 2001 (PubMed).

Leo, Chen: "The SRC family of nuclear receptor coactivators." in: Gene, Vol. 245, Issue 1, pp. 1-11, 2000 (PubMed).

Nakashima, Yanagisawa, Arakawa et al.: "Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300." in: Science (New York, N.Y.), Vol. 284, Issue 5413, pp. 479-82, 1999 (PubMed).

Mangelsdorf, Evans: "The RXR heterodimers and orphan receptors." in: Cell, Vol. 83, Issue 6, pp. 841-50, 1996 (PubMed).

Mangelsdorf, Thummel, Beato et al.: "The nuclear receptor superfamily: the second decade." in: Cell, Vol. 83, Issue 6, pp. 835-9, 1996 (PubMed).

Catalog No. ABIN123799
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